POTENTIALITY OF Quercus robur, L. FOR INDUCING SHOOTLETS MASS PRODUCTION THROUGH TISSUE CULTURE TECHNIQUES AND FACTORS INFLUENCING ITS SUCCESS

Gad,, Mervat M.; Shehata, M. S.

doi:10.21608/jssae.2003.246115

POTENTIALITY OF Quercus robur, L. FOR INDUCING SHOOTLETS MASS PRODUCTION THROUGH TISSUE CULTURE TECHNIQUES AND FACTORS INFLUENCING ITS SUCCESS

Document Type : Original Article

Authors

Forestry and Woody Trees Dept., Horticultural Research Institute, Agricultural Research Center, Giza, Egypt

10.21608/jssae.2003.246115

Abstract

This work was investigated in order to oblain mass production of snoouets
from Q. robur, as a promising tree. Ph.,,1CI exudates was a major problem, which was
overcome by manipulating the mother seedling by a dark system followed by handling
the explants obtained by successive steps. The effect of explant position on mother
seedling branch, medium type, plant growth regulators (type and concentration),
sucrose concentration, and the solidifying agent (agar and gerlite), were Investigated.
Favourable culture establishment was obtained by Inoculating the medial nodes
segments on WPM supplemented with 0.2 mg L" ISA + 0.8 mg L" SA rather than on
QL medium. A high frequency of shooUets multiplication (60/explant) was obtained on
WPM supplemented with 3% sucrose and 0.2 eng L'! ISA + 1.2 mg L·¹ SA. Successful
rooting of shooUets was induced by modifying WPM with 0.5 mg L" IBA and solidified
by 0.2% genite which surpassed 0.7% agar in rooting criteria.

Keywords