Mohamed, S. (2001). INFLUENCE OF DIFFERENT SOLVENTS ON THE QUALITY OF THE SOLUBILIZED PROTEIN. Journal of Soil Sciences and Agricultural Engineering, 26(9), 5813-5826. doi: 10.21608/jssae.2001.256597
Samira S. Mohamed. "INFLUENCE OF DIFFERENT SOLVENTS ON THE QUALITY OF THE SOLUBILIZED PROTEIN". Journal of Soil Sciences and Agricultural Engineering, 26, 9, 2001, 5813-5826. doi: 10.21608/jssae.2001.256597
Mohamed, S. (2001). 'INFLUENCE OF DIFFERENT SOLVENTS ON THE QUALITY OF THE SOLUBILIZED PROTEIN', Journal of Soil Sciences and Agricultural Engineering, 26(9), pp. 5813-5826. doi: 10.21608/jssae.2001.256597
Mohamed, S. INFLUENCE OF DIFFERENT SOLVENTS ON THE QUALITY OF THE SOLUBILIZED PROTEIN. Journal of Soil Sciences and Agricultural Engineering, 2001; 26(9): 5813-5826. doi: 10.21608/jssae.2001.256597
INFLUENCE OF DIFFERENT SOLVENTS ON THE QUALITY OF THE SOLUBILIZED PROTEIN
Fats and Oil Department, National Research Center, Dokki, Giza (Egypt)
Abstract
The proteins of soybean and cottonseed meals were extracted with different solvents, with the aim of examining their effects on the composition, antinutritional factors, toxic ingredients, digestibility and some functional properties of the extracted proteins products. The examined solvents included sodium hydroxide, sodium chloride, sodium carbonate, sodium bicarbonate and sodium carbonate/sodium bicarbonate buffers. Results show that sodium hydroxide 0.05 M was the best . It extracted highest protein from both investigated oilseed meals, it also resulted in highest reduction in the phytate content. Urease content of soybean was lowered to below the permissible level. The protein extracted with the sodium hydroxide possessed the highest digestibility compared to proteins extracted with other solvents. The functional properties of the extracted protein revealed a closeness to those of the original meals. The only disadvantage with 0.05 N sodium hydroxide is that it extracted very little gossypol from cottonseed meal resulting in a protein with 0.235% free gossypol. In this respect the carbonate/bicarbonate buffer pH 10.4 would be the choice solvent since it results in a protein product with 97% less gossypol than the original cottonseed meal.
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