PRODUCTION OF ENZYMES FROM WHEAT STRAW BY Aspergillus niger AND ASPERGILLUS TERREUS

Document Type : Original Article

Authors

Soils, Water and Environ. Res. Inst., Agric. Res. Center, Giza, Egypt.

Abstract

Aspergillus niger and Aspergillus terreus were tested for production of hydrolytic enzymes (cellulolytic enzymes, alpha-amylase and amyloglucosidase). Three different media of wheat straw were used. These occurred by cultivation of A. niger or A. terreus for 6 days with shaking (~ 120 rpm) at 30°C. A. terreus produced little higer quantities of alpha-amylase and amyloglucosidase than A. niger, but A. niger was superior for the production of cellulolytic enzymes. The maximum products of enzymes activities during cultivation of A. niger in medium III were 3.1 and 1.4 U/ml of alpha-amylase and amyloglucosidase ; 4.8 and 4.8 mg sugar / ml / 24 h of C1 and Av enzymes ; 8.9 and 16.4 mg sugar/ml/h of Cx and X enzymes, respectively and 3.3 mg/ml of reducing sugar.

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