Ali, S., Sharaf El-Deen, H., Eisa, S. (2006). SALINITY INDUCED DISSMILARITY AND AFFECTED GENE EXPRESSION IN SOME GRAMINACEOUS TAXA. Journal of Soil Sciences and Agricultural Engineering, 31(12), 8131-8150. doi: 10.21608/jssae.2006.225058
S.H. Ali; H. A. M. Sharaf El-Deen; S. S. Eisa. "SALINITY INDUCED DISSMILARITY AND AFFECTED GENE EXPRESSION IN SOME GRAMINACEOUS TAXA". Journal of Soil Sciences and Agricultural Engineering, 31, 12, 2006, 8131-8150. doi: 10.21608/jssae.2006.225058
Ali, S., Sharaf El-Deen, H., Eisa, S. (2006). 'SALINITY INDUCED DISSMILARITY AND AFFECTED GENE EXPRESSION IN SOME GRAMINACEOUS TAXA', Journal of Soil Sciences and Agricultural Engineering, 31(12), pp. 8131-8150. doi: 10.21608/jssae.2006.225058
Ali, S., Sharaf El-Deen, H., Eisa, S. SALINITY INDUCED DISSMILARITY AND AFFECTED GENE EXPRESSION IN SOME GRAMINACEOUS TAXA. Journal of Soil Sciences and Agricultural Engineering, 2006; 31(12): 8131-8150. doi: 10.21608/jssae.2006.225058
SALINITY INDUCED DISSMILARITY AND AFFECTED GENE EXPRESSION IN SOME GRAMINACEOUS TAXA
1Dept of Biochem. Fac. Agric. Am Shams Univ. Shoubra El4heima, Cairo, Egypt
2Dept of Botany, Fac. Agric. Am Shams Univ. Shoubra El4heima, Cairo, Egypt
Abstract
The present study was conducted in order to gain a better understanding of the significance of genetic factors and gene expression which are related to the effect of salt stress on the polypeptide levels in plants. To achieve this, the current work tested six Graminaceous taxa of sorghum and forage crops under three levels of salinity (50, loo and 150 mM NaCI). The varieties were examined morphologically, physiologically and at the level of molecular biology by SDSPAGE analysis. Results revealed that growth inhibition was observed in the plants subjected to 100 mM NaCI for 9 weeks while those subjected to 50 mM were not severely affected and still grow with lower rate till the end of experiment. On the other hand, all plants can’t continue to grow at the higher salinity level III. Only two of six varieties showed tolerant ability towards high sanity stress (level Il loo mM NaCI), one of these two varieties was not capable continuing alive under this leve! of salinity and the other could. Extractable sodium rose linearly with an increase in external NaCI concentration. The extractable sodium from plants treated with 50 and 100 mM NaCI were fold higher than ¡ri control plants. Dendrogram was used to identify changes that resulted when plants were grown in the presence of 50, 100 and 150 mM NaCI for 9 weeks. SDS-PAGE showed that protein patterns for control and salt stressed plant were changed as shown from Rf changing values and newly protein with lower molecular weight 16-30 kDa were found. This observation was noticeable markedly in variety Pearl millet at second level of saliruty. Dendrogram revealed dissimilarity attributed to salt stress with similarity coefficient varied due to salt stress effect. This result markedly showed between level I of salinity ¡n Pearl millet which give similarity coeffæient value in evel 1 close to control (0.86) and this Sc changed to 0.61 ¡n level 1 of salt stress for the same plant. Salinity showed different sc index between the treated plants and its control. The study suggests that salt to’erance is not conferred by a single trait, but is the consequence of complex gene interaction.
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