Benkhayal, F., AI-Saadi, M., AI-Saadi, A., AI-Sanousi, H. (2005). GC-MS, COUPLED OXIDATION ~CAROTENE AND DPPH' RADICAL OF Sa/via fruticosa VOLATILE OIL. Journal of Soil Sciences and Agricultural Engineering, 30(11), 7205-7213. doi: 10.21608/jssae.2005.238500
F. A. Benkhayal; M. H. AI-Saadi; A. H. AI-Saadi; H. M. AI-Sanousi. "GC-MS, COUPLED OXIDATION ~CAROTENE AND DPPH' RADICAL OF Sa/via fruticosa VOLATILE OIL". Journal of Soil Sciences and Agricultural Engineering, 30, 11, 2005, 7205-7213. doi: 10.21608/jssae.2005.238500
Benkhayal, F., AI-Saadi, M., AI-Saadi, A., AI-Sanousi, H. (2005). 'GC-MS, COUPLED OXIDATION ~CAROTENE AND DPPH' RADICAL OF Sa/via fruticosa VOLATILE OIL', Journal of Soil Sciences and Agricultural Engineering, 30(11), pp. 7205-7213. doi: 10.21608/jssae.2005.238500
Benkhayal, F., AI-Saadi, M., AI-Saadi, A., AI-Sanousi, H. GC-MS, COUPLED OXIDATION ~CAROTENE AND DPPH' RADICAL OF Sa/via fruticosa VOLATILE OIL. Journal of Soil Sciences and Agricultural Engineering, 2005; 30(11): 7205-7213. doi: 10.21608/jssae.2005.238500
GC-MS, COUPLED OXIDATION ~CAROTENE AND DPPH' RADICAL OF Sa/via fruticosa VOLATILE OIL
1Dept. of Food Science and Technology, College of Agric., University of Omar EL- Mukhtar, Libya
2Faculty of Pharmacy, University of Omar EL- Mukhtar, Libya
3Dept. of Zoology, College of Sci., Univ. of Omar EL- Mukhtar, Libya
4Dept. of Botany, College of Sci., Univ. of Omar EL- Mukhtar, Libya
Abstract
The volatile oil of Sa/via fruticosa which was extracted from the green leaves using hydrodistillation had a yellow color/aromatic and its percentage was 0.7% . The oil was studded for its constitutes and antioxidant activity . GC-MS results showed the presence of twenty three component , ten of them were found in high (1,8-Cineole ,1 ,8-Cineol Isomer ,Camphor, Trans-p-Caryophyllene .o-Plnene ,p-Myrcene, Linalool . a-Terpineol «x-Terpineol isomer, aCamphene) .
The volatile oil was separated on silica gel plates using Benzene:
Ethyl acetate (94:6 v/v) as a mobile phase. The separated bonds were sprayed by solution of p-carotene and linoleic acid, in order to determinate its antioxidant activity . only the bands of RF (0.21,0.35,0.93) showed an antioxidant effect. p-carotene bleaching test was used to measure the antioxidant effect and to compare its activity with BHT . The results revealed that a clear antioxidant activity of the oil when compared to blank.
In an attempt to understand the mechanism by which the oil act antioxidant , DPPH' free radical test was used . A weak antioxidant effect was obtained for the five increased concentration used in this study when compared to BHT at 25 and 50 ppm . this could be attributed to the fact that the oil contain compounds which act as antioxidant by different mechanisms rather than its donation of proton (H+)
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