GC-MS, COUPLED OXIDATION ~CAROTENE AND DPPH' RADICAL OF Sa/via fruticosa VOLATILE OIL

Benkhayal, F. A.; AI-Saadi, M. H.; AI-Saadi, A. H.; AI-Sanousi, H. M.

doi:10.21608/jssae.2005.238500

GC-MS, COUPLED OXIDATION ~CAROTENE AND DPPH' RADICAL OF Sa/via fruticosa VOLATILE OIL

Document Type : Original Article

Authors

¹ Dept. of Food Science and Technology, College of Agric., University of Omar EL- Mukhtar, Libya

² Faculty of Pharmacy, University of Omar EL- Mukhtar, Libya

³ Dept. of Zoology, College of Sci., Univ. of Omar EL- Mukhtar, Libya

⁴ Dept. of Botany, College of Sci., Univ. of Omar EL- Mukhtar, Libya

10.21608/jssae.2005.238500

Abstract

The volatile oil of Sa/via fruticosa which was extracted from the
green leaves using hydrodistillation had a yellow color/aromatic and its
percentage was 0.7% . The oil was studded for its constitutes and antioxidant
activity . GC-MS results showed the presence of twenty three component ,
ten of them were found in high (1,8-Cineole ,1 ,8-Cineol Isomer ,Camphor, Trans-p-Caryophyllene .o-Plnene ,p-Myrcene, Linalool . a-Terpineol
«x-Terpineol isomer, aCamphene) .
The volatile oil was separated on silica gel plates using Benzene:
Ethyl acetate (94:6 v/v) as a mobile phase. The separated bonds were
sprayed by solution of p-carotene and linoleic acid, in order to determinate its
antioxidant activity . only the bands of RF (0.21,0.35,0.93) showed an
antioxidant effect. p-carotene bleaching test was used to measure the
antioxidant effect and to compare its activity with BHT . The results revealed
that a clear antioxidant activity of the oil when compared to blank.
In an attempt to understand the mechanism by which the oil act antioxidant
, DPPH' free radical test was used . A weak antioxidant effect was obtained
for the five increased concentration used in this study when compared to BHT
at 25 and 50 ppm . this could be attributed to the fact that the oil contain
compounds which act as antioxidant by different mechanisms rather than its
donation of proton (H+)