Salim, A. (2002). THE USE OF LOW QUALITY SWEET POTATOES ROOTS (Ipomoea batatas) FOR ALPHA·AMYLASE PRODUCTION BY Aspergillus oryzae. Journal of Soil Sciences and Agricultural Engineering, 27(7), 5003-5012. doi: 10.21608/jssae.2002.255179
A.E.I. Salim. "THE USE OF LOW QUALITY SWEET POTATOES ROOTS (Ipomoea batatas) FOR ALPHA·AMYLASE PRODUCTION BY Aspergillus oryzae". Journal of Soil Sciences and Agricultural Engineering, 27, 7, 2002, 5003-5012. doi: 10.21608/jssae.2002.255179
Salim, A. (2002). 'THE USE OF LOW QUALITY SWEET POTATOES ROOTS (Ipomoea batatas) FOR ALPHA·AMYLASE PRODUCTION BY Aspergillus oryzae', Journal of Soil Sciences and Agricultural Engineering, 27(7), pp. 5003-5012. doi: 10.21608/jssae.2002.255179
Salim, A. THE USE OF LOW QUALITY SWEET POTATOES ROOTS (Ipomoea batatas) FOR ALPHA·AMYLASE PRODUCTION BY Aspergillus oryzae. Journal of Soil Sciences and Agricultural Engineering, 2002; 27(7): 5003-5012. doi: 10.21608/jssae.2002.255179
THE USE OF LOW QUALITY SWEET POTATOES ROOTS (Ipomoea batatas) FOR ALPHA·AMYLASE PRODUCTION BY Aspergillus oryzae
Dept. of Agrlc. Mlcroblol., Fac. of Agrlc., Mansoura Un Iv., Egypt.
Abstract
Low quality sweet potatoes roots were used as a carbon source to produce extracellular alpha-amylase by Asperigiffus arrzae in shake flask cultures. Peak alpha - amylase production was obtained by local strain of Aspergillus oryzae on sweet potatoes filtrate medium amended with saHs solutions and sodium nitrate, pH 6.0 after incubation at 3Q·C for 5 days. The optimum temperature and pH of the enzyme activity was 50"C and 5.6, respectively. The optimum time for enzyme actiVity, using starch buffered 0.5%, was 1h at optimum pH 5.6 and optimum temperature 50·C. The produced enzyme is stable at temperatures below 50·C, while the enzyme lost 13.1, 24.5,46.8 and 57.2% of its original activity after exposure at 55, 60,65 and 75·C for 1h., respectively.
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