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Journal of Soil Sciences and Agricultural Engineering
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Mohamed, S. (2000). UTILIZATION OF COTTON STALKS. Journal of Soil Sciences and Agricultural Engineering, 25(7), 4777-4783. doi: 10.21608/jssae.2000.259456
Sekina A. Mohamed. "UTILIZATION OF COTTON STALKS". Journal of Soil Sciences and Agricultural Engineering, 25, 7, 2000, 4777-4783. doi: 10.21608/jssae.2000.259456
Mohamed, S. (2000). 'UTILIZATION OF COTTON STALKS', Journal of Soil Sciences and Agricultural Engineering, 25(7), pp. 4777-4783. doi: 10.21608/jssae.2000.259456
Mohamed, S. UTILIZATION OF COTTON STALKS. Journal of Soil Sciences and Agricultural Engineering, 2000; 25(7): 4777-4783. doi: 10.21608/jssae.2000.259456

UTILIZATION OF COTTON STALKS

Article 10, Volume 25, Issue 7, July 2000, Page 4777-4783  XML PDF (375.16 K)
Document Type: Original Article
DOI: 10.21608/jssae.2000.259456
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Author
Sekina A. Mohamed
Soils, Water and Environ. Res. Inst., Agric. Res. Center, Giza, Egypt.
Abstract
Three different methods has been used for the hydrolysis of the cotton stalks to reducing sugar and valuable products. These methods involves: (1) Biological degradation by cultivation of Aspergillus niger on cotton stalks in simple media. The bio-products of A. niger growth in cultures filtrate were evaluated after 6 days of incubation with shaking (~120 rmp) at 300C. (2) Dual-step acid hydrolysis (H2SO4 72%, 4%, v/v) at different time and temperature of hydrolysis.(3)- Enzymatic hydrolysis (two sources of cellulases, separately) for 3 days at 370C and pH 4.6. In biological degradation, the highest bioproducts in culture filtrate (1.72 mg/ml reducing sugar; 5.01 and 1.27 U/ml of µ - amylase and amyloglucosidase; 5.92 and 9.31 mg sugar / ml / h of Cx and X enzymes and 2.78 and 2.37 mg sugar / ml / 24h of C1 and Av enzymes, respectively) were obtained in medium was contained: 10 g cotton stalks, 10g CaCO3 and 100 ml water. In dual step acid hydrolysis, the high yield of reducing sugar (80.0 %) was obtained with 85 min - first step acid hydrolysis and 60 min second step acid hydrolysis. While, high yield of reducing sugar in enzymatic hydrolysis (14.97 mg / ml) was occurred with solid cellulase.
Keywords
cotton stalks; Aspergillus niger; acid hydrolysis; enzymatic hydrolysis; low-cost media
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