Three different methods has been used for the hydrolysis of the cotton stalks to reducing sugar and valuable products. These methods involves: (1) Biological degradation by cultivation of Aspergillus niger on cotton stalks in simple media. The bio-products of A. niger growth in cultures filtrate were evaluated after 6 days of incubation with shaking (~120 rmp) at 300C. (2) Dual-step acid hydrolysis (H2SO4 72%, 4%, v/v) at different time and temperature of hydrolysis.(3)- Enzymatic hydrolysis (two sources of cellulases, separately) for 3 days at 370C and pH 4.6. In biological degradation, the highest bioproducts in culture filtrate (1.72 mg/ml reducing sugar; 5.01 and 1.27 U/ml of µ - amylase and amyloglucosidase; 5.92 and 9.31 mg sugar / ml / h of Cx and X enzymes and 2.78 and 2.37 mg sugar / ml / 24h of C1 and Av enzymes, respectively) were obtained in medium was contained: 10 g cotton stalks, 10g CaCO3 and 100 ml water. In dual step acid hydrolysis, the high yield of reducing sugar (80.0 %) was obtained with 85 min - first step acid hydrolysis and 60 min second step acid hydrolysis. While, high yield of reducing sugar in enzymatic hydrolysis (14.97 mg / ml) was occurred with solid cellulase.
Mohamed, S. (2000). UTILIZATION OF COTTON STALKS. Journal of Soil Sciences and Agricultural Engineering, 25(7), 4777-4783. doi: 10.21608/jssae.2000.259456
MLA
Sekina A. Mohamed. "UTILIZATION OF COTTON STALKS", Journal of Soil Sciences and Agricultural Engineering, 25, 7, 2000, 4777-4783. doi: 10.21608/jssae.2000.259456
HARVARD
Mohamed, S. (2000). 'UTILIZATION OF COTTON STALKS', Journal of Soil Sciences and Agricultural Engineering, 25(7), pp. 4777-4783. doi: 10.21608/jssae.2000.259456
VANCOUVER
Mohamed, S. UTILIZATION OF COTTON STALKS. Journal of Soil Sciences and Agricultural Engineering, 2000; 25(7): 4777-4783. doi: 10.21608/jssae.2000.259456
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